|Year : 2017 | Volume
| Issue : 4 | Page : 424-431
Management of difficult hepatic artery anastomosis in living donor liver transplantation: mansoura experience
Ayman El Nakeeb MD , Mohamed El Shobary, Tarek Salah, Ayman El Nakeeb, Ahmad M Sultan, Ahmed Elghawalby, Mohamed Abdel Wahab
General Surgery Department, Gastroenterology Surgical Center, Mansoura University, Mansoura, Egypt
|Date of Submission||02-Jul-2017|
|Date of Acceptance||09-Aug-2017|
|Date of Web Publication||13-Nov-2017|
Ayman El Nakeeb
General Surgery Department, Gastroenterology Surgical Center, Mansoura University, Mansoura 35516
Source of Support: None, Conflict of Interest: None
One of the most difficult and important procedure in living donor liver transplantation (LDLT) is hepatic artery reconstruction. Difficult hepatic artery reconstruction may be because of pathological factor such as intimal dissection (ID) and anatomical variation. Difficult hepatic artery reconstruction is a risk factor for hepatic artery complications. This study was done to evaluate difficult hepatic artery reconstruction in LDLT at our center and its surgical outcomes.
Patient and methods
Consecutive patients who were treated for end-stage liver cirrhosis by LDLT were retrospectively reviewed. The management of hepatic artery with ID is carried out according to the extent of ID.
Hepatic artery ID was found in 21/375 (5.6%) cases. Overall, seven (33%) cases were reconstructed with the graft hepatic artery after trimming the edge until reaching a healthy segment. A total of 11 (52.4%) cases were reconstructed with the graft hepatic artery after intimal fixation of ID. Moreover, three (14.3%) cases had severe ID and failed intimal fixation and were reconstructed with the recipient splenic artery. Biliary stricture developed in two patients who had severe ID, and three patients developed transient bile leak. No hepatic artery complications, graft failure, or mortality occurred.
Intimal fixation technique proved to be an effective technique in most of the cases, with good short-term and long-term follow-up results. In severe ID or failure of intimal fixation, alternative recipient arteries other than hepatic artery can be used.
Keywords: biliary stricture, hepatic artery, intimal dissection, living donor transplant
|How to cite this article:|
El Nakeeb A, El Shobary M, Salah T, El Nakeeb A, Sultan AM, Elghawalby A, Wahab MA. Management of difficult hepatic artery anastomosis in living donor liver transplantation: mansoura experience. Egypt J Surg 2017;36:424-31
|How to cite this URL:|
El Nakeeb A, El Shobary M, Salah T, El Nakeeb A, Sultan AM, Elghawalby A, Wahab MA. Management of difficult hepatic artery anastomosis in living donor liver transplantation: mansoura experience. Egypt J Surg [serial online] 2017 [cited 2018 Jun 21];36:424-31. Available from: http://www.ejs.eg.net/text.asp?2017/36/4/424/218171
| Introduction|| |
Living donor liver transplantation (LDLT) has obtained worldwide agreement, especially in countries that lacked deceased donors ,,. In many countries, the use of deceased donor liver transplantation (LT) has not been approved legally. Therefore, LDLT remains the only hope for management of patients with end stage liver disease (ESLD) ,,. One of the most difficult and important procedures in LDLT is reconstruction of the hepatic artery (HA) because the HA of the graft is usually short and small in diameter ,,. Moreover, the recipient artery is occasionally damaged and adds a more difficult scenario to an already existing one ,.
HA complications, including HA stenosis, hepatic artery thrombosis (HAT), bleeding from the anastomotic site, and rupture pseudoaneurysm, during LT can lead to increased morbidity and mortality in liver transplant recipients, and a retransplantation may be required in most of the cases ,. The incidence of HA complications is 1.6–8% in adult recipients and 2–23.6% in pediatric recipients ,,,. The causes of HA complications are multifactorial including technical, anatomical, pediatric LT, intimal dissection (ID), and pathological factors ,,,,,. The incidence of HAT has reduced in recent years owing to improvement in surgical techniques, postoperative anticoagulants, and radiological modalities ,.
ID is one of the risk factors of HA complications. The intima of HA may be injured during dissection, during transarterial embolization (TAC), or because of atherosclerosis ,,,,. The ID was classified according to the extent of intimal injury into three grades: mild ID, when the extent of ID was less than one-quarter of the circumference of the HA; moderate ID, when it involved one-half of the circumference of the artery of the HA; and severe ID, when the extent of ID reached more than one-half of the circumference of the HA or the entire vessel wall ,. The management of ID is performed according to the extent of intimal injury, by either trimming the edge until a healthy part of the artery is reached, fixation of the intima, or by using alternative vessels to replace the injured native HA ,,,,,. The results of intimal fixation for ID of HA have not been fully elucidated. This study was done to evaluate the incidence and different management techniques of ID of HA and its outcome.
| Patients and methods|| |
Consecutive patients, who were treated for end-stage liver cirrhosis by LDLT at Gastroenterology Surgical Center, Mansoura University, Egypt, during the period from April 2004 to April 2015, were retrospectively reviewed. In this period, three cases were done for children who received the left lobe graft from their mothers. All data were reviewed from a prospectively maintained database on internal web-based registry system completed by paper records.
Donor selection and workup
A multistep, multidisciplinary protocol was used for donor evaluation in our center, and it has been described elsewhere ,.
Initial evaluation includes complete blood count (CBC), electrolytes, liver function tests (LFT), coagulation profile, viral serology, AFP, ABO blood typing, C-reactive protein, and renal function tests. The model for end-stage disease score was calculated. Radiographic studies including abdominal ultrasound (US) and triphasic computed tomography were used to assess the liver status and in case of hepatocellular carcinoma (HCC) to assess the number and size of the tumor.
Surgery was performed through a right subcostal incision with midline extension. The operative details in our center were described elsewhere ,. Overall, 100 IU/kg heparin was injected before HA clamping. The liver graft was flushed immediately with cold histidine-tryptophan-ketoglutarate solution through the portal vein on the back table. Cannulation and flushing of the artery was not performed to avoid damage to the intima of the HA. The diameter of middle hepatic vein (MHV) tributaries was rechecked on the back table, and those with a diameter of more than 5 mm were considered for reconstruction.
The abdomen was entered through a low subcostal incision with midline extension in all cases. The abdomen was thoroughly examined for any extrahepatic metastasis missed on preoperative imaging in cases with HCC. The liver was mobilized by dissecting all its ligaments. Hilar dissection was then approached with minimal dissection between arterial and biliary systems. Total hepatectomy was performed, preserving the inferior vena cava (IVC). The left hepatic vein (LHV) stump or the MHV/LHV stump was closed in most of the cases or used for MHV reconstructions.
The right hepatic vein (RHV) plasty was done routinely. The RHV of the patient and the liver graft are anastomosed in an end-to-end fashion using running 4/0 prolene sutures, leaving a loose stitch for venting of blood. Portal vein reconstruction was done using prolene 6-0. After venting of 300 ml of blood, the loose suture in the hepatic venous anastomosis was tied and the graft was reperfused.
Hepatic arterial reconstruction was performed using interrupted 8/0 Prolene sutures under 4.5 loupe magnification using midposterior wall first technique. Our policy is to anastomose either the right or left branch of the HA to the donor artery.
The management of HA with ID is carried out according to the extent of intimal injury; in cases with mild or moderated ID, trimming the edge was done until reaching a healthy part of the artery. Intimal fixation was performed with either 1, 2, 3 or even 4 quadrants and then proceed to anastomosis in cases of moderate or severe ID. We use double needle 6-mm 8/0 Prolene suture from inside out 2 mm away from the edge to fix the intima to adventitia to facilitate the anastomosis, and then ligation outside ([Figure 1]a, [Figure 1]b, [Figure 1]c). All interrupted stitches of the anastomosis were carried out from the inside of the artery to the outside. The total number of stitches range from eight to 12 according to the arterial caliber. The recipient artery used for reconstruction were hepatic arteries, except in a few cases where the HA was severely damaged, in which the splenic artery was used for reconstruction ([Figure 2]). Intraoperative Doppler was done for all cases at the end of the procedure to assess the vascular patency and blood flow in RHV, portal vein (PV), and HA. Duct-to-duct biliary anastomosis is performed using 6/0 polydioxanone suture (PDS) or Maxon 6/0 in an interrupted fashion, over an indwelling 4–5 Fr catheter. This stent emerges from either CBD or cystic duct stump. Three drains were placed in right subphrenic space, cut margin of the graft, and pelvis.
|Figure 1 (a–c) Intimal fixation was performed with either 1, 2, 3 or even 4 quadrants using double needle 6 mm 8/0 prolene suture from inside out, 2 mm away from the edge then ligation outside.|
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All patients were managed in the ICU before transfer to the ward. All patients received prophylactic antibiotics intraoperatively and postoperatively. Anticoagulant was not given postoperatively. Routinely all patients were given intravenous infusion of magnesium and phosphate until tolerating diet. The drain was removed if the amount was less than100 ml/day after 1 week and if there was no bile leak, or pus. Complete blood picture, liver function tests, serum electrolytes, creatinine and C-reactive protein findings were monitored daily.
Vascular patency was followed up using Doppler ultrasonography (DUS) daily for 2 weeks, weekly till discharge, and then monthly till 6 months. Computed tomography angiography was performed 6 months after LT ([Figure 3]). A measurable velocity flow on DUS was considered an indicator of patency.
|Figure 3 Angiography revealing patent hepatic artery was performed 6 months after liver transplantation after IF.|
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Immunosuppressive treatment started intraoperatively after arterial reconstruction and consisted of steroid and Simulect. The regimen consisted of tacrolimus and cellcept from the fourth day postoperatively. Initial rejection was treated by adding steroid.
The decision of discharge from hospital was based on patient’s general condition, clinical parameters, absence of complication, and imaging study.
Follow-up and data collection
Donors and recipient were followed up after hospital discharge with laboratory investigation, abdominal US, and Magnetic Resonance Cholangiopancreatography (MRCP) in selected cases every month for the first month, then every 6 months, and then every year postoperatively. Follow-up visits included clinical examination, laboratory investigation, doses of immunosuppressive, radiological examination, and DUS. All preoperative, operative, and postoperative data were recorded retrospectively from a prospectively maintained database on internal web-based registry system completed by paper records.
Continuous variables are expressed as median. Categorical variables were expressed as numbers and percentages. Analysis was performed with SPSS program 17 for Windows (SPSS Inc., Chicago, Illinois, USA). Comparison of variables was done by independent student t-test for continuous variables and χ2-test for categorical variables. A P value less than 0.05 was considered statistically significant. Variables with P less than 0.1 were entered into a logistic regression model to determine independent risk factors for postoperative complications.
| Results|| |
From May 2004 to July 2015, 375 cases of living donor liver transplantation (LDLTx) for adult were done in Gastroenterology Surgical Center, Mansoura University, Egypt. Single arterial anastomosis was done in 372 cases, and double arterial anastomosis was performed in three cases. The median diameter of donor HA is 2 mm (1–3 mm). A total of 326 cases were anastomosed to right HA branch and 49 to left HA branch. Preoperative data of patients with HA ID are shown in [Table 1] and [Table 2].
HA ID was found in 21 (5.6%) cases. Trimming of the edge of recipient HA was performed in five cases had mild ID and two cases had moderate ID and were reconstructed with graft HA. Revision of anastomosis was carried out in the same setting in four cases of them, as DUS showed no flow. Intimal fixation using 8/0 Prolene double needle of 6 mm was done in 11 (52.4%) cases (six cases had moderate ID and five cases had severe ID). Intimal fixation was done as four fixation sutures in five cases, three fixation sutures in one case, and the remaining five cases by two fixation sutures. Revision of anastomosis was performed after intimal fixation (IF) in two cases of them, as DUS showed no flow. Trial splenic artery replacement was done in four cases when HA was severely damaged and was successful in three of them. In case of failed splenic artery replacement because splenic artery, in this case, was short and unsuitable, soothe left HA was used after IF ([Table 3],[Table 4],[Table 5]).
All cases showed excellent Doppler wave intraoperatively, with average resistive index of 0.69. Long-term follow-up using Doppler and in suspected cases computed tomography angiography showed good patency in all cases. HAT, stenosis, or pseudoaneurysm did not occur in any case. No graft failure or mortality occurred.
A total of five patients developed biliary complications. Biliary stricture developed in two patients who had severe ID and was managed by IF: one of them requiring seven sets of ERCP for dilatation and US-guided tubal drainage for the biliary collection, and the other one developed very tight stricture and failed to pass the guide wire by ERCP, so hepaticojejunostomy was done for him. Moreover, three patients (one had severe ID and was managed by IF, the second had severe ID and was managed by splenic artery replacement, and the third had mild ID and was treated by trimming the edge) developed transient bile leak; two of them were managed conservatively and one by ERCP ([Table 4] and [Table 5])
The severity of ID affected the surgical outcome and development of biliary complications, as four of five cases that developed biliary complications had severe ID (P=0.06) ([Table 5] and [Table 6]).
|Table 6 Univariate and multivariate analyses of variables affecting postoperative surgical outcome|
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| Discussion|| |
HA reconstruction is a challenging point in LDLT because the artery has small caliber and short stump and is a technically complicated procedure ,,,. Complications of HA reconstruction are one of the leading causes of graft failure and mortality after LDLT. HA complication after LDLT includes HAT, HA stenosis, and HA pseudoaneurysm ,,,,,,. The early complications of HA are usually because of technical, anatomical, or pathological factors including small diameter, pediatric recipient, prolonged clamping of the artery, kinking of a long artery, hematoma of the artery wall, severe hypotension, prolonged cold ischemia, acute rejection, and ID ,,,,.
ID is one of the risk factors of development of complications after HA reconstruction,,. The intima of HA may be injured owing to atherosclerosis, prolonged clamping, vigorous traction, and grasping of HA during dissection or during pretransplant TAC for hepatocellular carcinoma ,,,,. To minimize the incidence of ID, careful atraumatic dissection and preparation of artery is mandatory. The management of ID is performed according to the extent of intimal injury, by either trimming the edge until a healthy part of artery is reached, fixation of the intima, or by using alternative vessels to replace the injured native HA including splenic, right gastroepiploic, left gastric, or gastroduodenal artery ,,,,,.
Lin et al.  classified the ID into three grades according to the extent of ID, and their management depended on the grade of ID. In mild and moderate ID, the HA can be used after trimming the edge till reaching a healthy part, but in severe ID, alternative recipient arteries other than HA were used. They found that 23 of 40 (57.5%) patients developed ID after TAC. Overall, nine patients had mild ID and six had moderate ID, and they were managed by trimming of the edge; eight had severe ID, and they were managed by alternative vessels other than HA. Two incidences of HAT developed postoperatively. No graft failure or mortality occurred.
Banshodani et al.  reported that when ID occurred, intimal fixation was performed at four points, and all stitches were carried out from inside to outside of the artery to facilitate good intimal fixation (intraluminal stitching technique). Intraoperative DUS was used to confirm adequate blood flow. Postoperative anticoagulant was given for 3 days. This technique was used in eight cases with ID, except in two cases with severe ID, and the recipient’s right gastroepiploic artery was used. Reanastomosis in primary operation was done in three cases, thrombectomy using Fogarty catheter was performed in one case, and thrombolytic agent was used in one case.
For ideal arterial reconstruction, careful dissection and preparation of recipient artery with intimal adaptation must be done. When there is neither an atherosclerotic change nor ID, each stitch is performed from the outside to the inner side of the artery using an 8/0 Prolene suture. In case of ID, double needle 6-mm 8/0 Prolene suture was used from inside out to fix the intima to adventitia, and then ligation outside. All interrupted stitches of the anastomosis were carried out from the inside of the artery to the outside ,. In the present study, five cases had mild ID and two cases hade moderate ID, and they were reconstructed with the graft HA after trimming the edge till reaching a health segment. Moreover, six cases had moderate ID and five cases had severe ID, and they were reconstructed with the graft HA after intimal fixation of dissected HA. A total of three cases had severe ID and failed intimal fixation and were reconstructed with the recipient splenic artery.
ID of HA may be a risk factor that leads to severe arterial complications such as HAT and subsequent graft failure and mortality ,,. In recent years, the arterial complications rate in LDLT was reduced to 3.5–6% owing to improvement of techniques, careful atraumatic dissection and preparation of the artery, introduction of microsurgical techniques ,,,,. Early diagnosis of HA complications based on serial DUS, even in asymptomatic patients, during the first 14 days is very important to decrease the incidence of biliary complications and graft failure because of early intervention. Urgent thrombectomy and revascularization have replaced retransplantation for early HAT ,,,.
Iida et al.  reported that HA complications occurred in 43 (6.4%) of 673 adult recipient. Postoperative biliary complications after hepatic arterial complications were found in 17 (39.5%) of the 43 cases. They were seven patients who developed biliary leak, six had hepatic pyogenic abscesses, and four patients had biliary strictures. Wang et al.  reported that five patients of 126 patients required an HA alternative using gastric arteries owing to ID of the recipient HA, which was found during primary transplant. Biliary complications developed in three patients: one patient had biliary stricture requiring percutaneous biliary drainage and two patients had bile leak. The indication of one bile leak recipient resolved conservatively. The other one had graft failure owing to leak and sepsis. In our study, five patients developed biliary complications. Biliary stricture was developed in two patients. One of them required seven sets of ERCP for dilatation. The other one developed very tight stricture and failed to pass the guide wire by ERCP, so hepaticojejunostomy was done for him. Three patients developed transient bile leak, two of them managed conservatively and one by ERCP.Several studies found that extra-anatomical HA reconstructions are strategy in cases with severe ID of HA and can save hepatic graft using other arteries, such as recipients’ gastric arteries, gastroduodenal artery, and splenic artery, instead of unusable HA ,,,. However, the anatomical anastomosis must be the first choice for the HA reconstruction because extra-anatomical HA anastomosis was a risk factor for development of HA complications, biliary complications, and for taking longer time ,,,,. When using splenic artery, ligation of the distal part of the artery may lead to splenic infarction in some cases . In the current study, trial splenic artery replacement was done in four cases with severe ID and was successful in three of them. Biliary complication developed in one case of them in the form of transient bile leakage and passed conservatively.
| Conclusion|| |
ID is one of the risk factors of development of complications after HA reconstruction in LDLT. To minimize the incidence of ID, careful atraumatic dissection and preparation of the artery is mandatory. Intimal fixation technique proved to be a simple and effective technique in most cases, with good short- and long-term follow-up and decreased shift to extra-anatomical reconstruction. In severe ID or failure of intimal fixation, alternative recipient arteries other than HA can be used.
Mohamed El Shobary and Ayman El Nakeeb designed the research. Mohamed El Shobary, Tarek Salah, Ayman El Nakeeb, Ahmad M. Sultan, Ahmed Elghawalby, and Mohamed A. Wahab performed the research. Mohamed El Shobary, Ayman El Nakeeb, and Ahmed M. Sultan analyzed the data. Mohamed El Shobary and Ayman El Nakeeb wrote the paper.
Financial support and sponsorship
Conflicts of interest
There are no conflicts of interest.
| References|| |
Maluf DG, Stravitz RT, Cotterall AH, Posner MP, Nakatsuka M, Sterling RK et al.
Adult living donor versus deceased donor liver transplantation: a 6-year single center experience. Am J Transplant 2005; 5:149–156.
Makuuchi M, Miller CM, Olthoff K, Schwartz M. Adult-adult living donor liver transplantation. J Gastrointest Surg 2004; 8:303–312.
Trotter JF, Wachs M, Everson GT, Kam I. Adult-to-adult transplantation of the right hepatic lobe from a living donor. N Engl J Med 2002; 346:1074–1082.
Salah T, Sultan MA, Fathy MO, Elshobary MM, Elghawalby AN, Sultan A et al.
Outcome of right hepatectomy for living liver donors: a single Egyptian center experience. J Gastrointest Surg 2012; 16:1181–1188.
Abdel Wahab MA, Hamed H, Salah T, Elsarraf W, Elshobary M, Sultan MA et al.
Problem of living liver donation in the absence of deceased liver transplantation program: Mansoura experience. World J Gastroenterol 2014; 20:13607–13614.
Sanada Y, Wakiya T, Hishikawa S, Hirata Y, Yamada N, Okada N et al.
Risk factors and treatments for hepatic arterial complications in pediatric living donor liver transplantation. J Hepatobiliary Pancreat Sci 2014; 21:463–472.
Marubashi S, Kobayashi S, Wada H, Kawamoto K, Eguchi H, Doki Y et al.
Hepatic artery reconstruction in living donor liver transplantation: risk factor analysis of complication and a role of MDCT scan for detecting anastomotic stricture. World J Surg 2013; 37:2671–2677.
Miyagi S, Kawagishi N, Nakanishi W, Fujio A, Miyazawa K, Maida K et al.
Risk factors for hepatic artery thrombosis after microsurgical vascular reconstruction in liver transplantation. Transplant Proc 2013; 45:1994–1996.
Banshodani M, Tashiro H, Onoe T, Ide K, Ohdan H. Long-term outcome of hepatic artery reconstruction during living-donor liver transplantation. Transplant Proc 2011; 43:1720–1724.
Li PC, Jeng LB, Yang HR, Lee CC, Poon KS, Chen TH et al.
Hepatic artery reconstruction in living donor liver transplantation: running suture under surgical loupes by cardiovascular surgeons in 180 recipients. Transplant Proc 2012; 44:448–450.
Yang Y, Zhao JC, Yan LN, Ma YK, Huang B, Yuan D et al.
Risk factors associated with early and late HAT after adult liver transplantation. World J Gastroenterol 2014; 20:10545–10552.
Iida T, Kaido T, Yagi S, Hori T, Uchida Y, Jobara K et al.
Hepatic arterial complications in adult living donor liver transplant recipients: a single-center experience of 673 cases. Clin Transplant 2014; 28:1025–1030.
Tannuri AC, Monteiro RF, Santos MM, Miyatani HT, Tannuri U. A new simplified technique of arterial reconstruction in pediatric living-donor liver transplantation: a comparison with the classical technique. J Pediatr Surg 2014; 49:1518–1521.
Okazaki M, Asato H, Takushima A, Nakatsuka T, Sarukawa S, Inoue K et al.
Hepatic artery reconstruction with double-needle microsuture in living-donor liver transplantation. Liver Transpl 2006; 12:46–50.
Lin TS, Chiang YC, Chen CL, Concejero AM, Cheng YF, Wang CC et al.
Intimal dissection of the hepatic artery following transarterial embolization for hepatocellular carcinoma: an intraoperative problem in adult living donor liver transplantation. Liver Transpl 2009; 15:1553–1556.
Piskin T, Demirbas T, Yalcin L, Yaprak O, Dayangac M, Guler N et al.
Recipient splenic artery utilization for arterial re-anastomosis in living donor liver transplantation: single-center experience. Hepatogastroenterology 2012; 59:1263–1264.
Uchiyama H, Shirabe K, Taketomi A, Soejima Y, Ninomiya M, Kayashima H et al.
Extra-anatomical hepatic artery reconstruction in living donor liver transplantation: can this procedure save hepatic grafts? Liver Transpl 2010; 16:1054–1061.
Takatsuki M, Chiang YC, Lin TS, Wang CC, Concejero A, Lin CC et al.
Anatomical and technical aspects of hepatic artery reconstruction in living donor liver transplantation. Surgery 2006; 140:824–828.
Wang CC, Lin TS, Chen CL, Concejero AM, Iyer SG, Chiang YC. Arterial reconstruction in hepatic artery occlusions in adult living donor liver transplantation using gastric vessels. Surgery 2008; 143:686–690.
Matsuda H, Yagi T, Sadamori HV. Complications of arterial reconstruction in living donor liver transplantation: a single-center experience. Surg Today 2006; 36:245–251.
Uchiyama H, Hashimoto K, Hiroshige S. Hepatic artery reconstruction in living-donor liver transplantation: a review of its techniques and complications. Surgery 2002; 131(Suppl):S200– S204.
D’albuquerque LA, Gonzalez AM, Letrinda RF. Use of the splenic artery for arterial reconstruction in living donor liver transplantation. Transplant Proc 2007; 39:3202.
Figueras J, Pares D, Aranda H. Results of using the recipient’s splenic artery for arterial reconstruction in liver transplantation in 23 patients. Transplantation 1997; 64:655.
[Figure 1], [Figure 2], [Figure 3]
[Table 1], [Table 2], [Table 3], [Table 4], [Table 5], [Table 6]